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目的：研究卵巢癌病人基因组不稳定和转化生长因子β受体Ⅱ基因（ＴβＲ—Ⅱ）突变与其发生关系。方法：应用聚合酶链反应技术和单链构象多态性分析方法，分析Ｄ２Ｓ１２３、Ｍｓｅ５和ＴβＲ—Ⅱ三个位点微卫星变化，并检测ＴβＲ—Ⅱ突变。结果：１５例卵巢癌中有３例表现微卫星不稳定，有２例检出ＴβＲ—Ⅱ突变。结论：部分卵巢癌病人基因组存在微卫星不稳定，与其发生有关。ＴβＲ—Ⅱ基因突变，使细胞失去对转化生长因子β的反应而发生肿瘤，可能为卵巢癌发生的分子机理之一
Objective: To study the relationship between genomic instability and the mutation of transforming growth factor β receptor Ⅱ (TβR-Ⅱ) in ovarian cancer patients. Methods: The microsatellite changes of D2S123, Mse5 and TβR-Ⅱ were analyzed by polymerase chain reaction and single-strand conformation polymorphism analysis, and the mutation of TβR-Ⅱ was detected. Results: Three of the 15 ovarian cancers showed microsatellite instability, and two cases of TβR-Ⅱ mutation were detected. Conclusion: Some ovarian cancer patients have microsatellite instability in their genome, which is related to their occurrence. TβR-Ⅱ gene mutation, so that cells lose the response to transforming growth factor β and tumor, may be one of the molecular mechanisms of ovarian cancer