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目的:观测中药肝复健冲剂对二乙基亚硝胺(DEN)诱导大鼠肝纤维化形成的影响.方法:建立DEN诱导大鼠肝硬化模型,同时给予肝复健冲剂进行干预,应用天狼红苦味酸特染法、免疫组化染色法及计算机图像分析技术检测中药对DEN致大鼠肝纤维化过程胶原沉淀及肝细胞增殖特性的影响.结果:随着模型大鼠肝纤维化程度加重,肝内胶原沉积量相应逐渐增加.中药组大鼠肝脏炎性损害及肝纤维化程度均较同期模型组大鼠轻,胶原沉积量及细胞增殖核抗原(PCNA)阳性着色肝细胞数明显减少,两组间差异有统计学意义,[胶原沉积量:4周(15.20±2.20)vs(7.19±0.96);12周(27.1±4.5)vs(16.0±1.6)。阳性表达PCNA肝细胞数:12周(21.1±0.31)vs(6.4±0.1);16周(31.1±7.0)vs(10.1±0.7)]。结论:中药肝复健冲剂可通过减少肝内胶原沉积及可能恶性增殖的肝细胞,抑制和延缓实验性动物肝纤维化的形成,并一定程度上预防肝硬化向肝癌的转变.
OBJECTIVE: To observe the effects of Ganfukang Granules on the formation of hepatic fibrosis induced by diethylnitrosamine (DEN) in rats.Methods: The model of liver cirrhosis induced by DEN was established in rats, Wolfberry picric acid special staining method, immunohistochemical staining and computer image analysis technology to detect the DEN-induced liver fibrosis in rat collagen deposition and hepatocyte proliferation characteristics.Results: With the model of liver fibrosis And the amount of intrahepatic collagen deposition gradually increased.The degree of hepatic inflammatory injury and hepatic fibrosis in the TCM group were lighter than those in the model group at the same time, and the deposition of collagen and the number of PCNA positive stained hepatocytes were significantly The difference was statistically significant between the two groups [collagen deposition: 4 weeks (15.20 ± 2.20) vs (7.19 ± 0.96); 12 weeks (27.1 ± 4.5) vs (16.0 ± 1.6). The number of PCNA positive cells was 12 weeks (21.1 ± 0.31) vs (6.4 ± 0.1); 16 weeks (31.1 ± 7.0) vs (10.1 ± 0.7). CONCLUSION: Ganfukang Granule can inhibit and delay the formation of hepatic fibrosis in experimental animals by reducing the intrahepatic collagen deposition and possible malignant proliferation of hepatocytes, and to a certain extent, prevent the cirrhosis from transforming into hepatocellular carcinoma.