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目的了解宁波市志贺菌血清型变化、耐药性及菌株毒力,为预防控制菌痢提供实验依据。方法从腹泻患者中分离菌株;鉴定采用API生化条;血清分型采用玻片凝集法;药敏采用VITEK 2 Compact GPS-16卡片和K-B法;5种毒力基因检测采用PCR法。结果 254株志贺菌中分出福氏志贺菌(B群)170株(占66.93%),宋内志贺菌(D群)83株(32.68%),鲍氏志贺菌(C群)1株(0.39%),其生化结果典型与ATCC标准株一致。志贺菌ipa H、set1A、set1B、sen、ial和vir A毒力基因携带率分别为100.00%、80.71%、75.20%、85.04%、94.49%和88.98%。耐药率前3位的是奎诺酮类的萘啶酸、四环素类的四环素和青霉素类的氨苄西林,部分菌株对三代头孢菌素中的头孢他啶、四代头孢菌素中的头孢吡肟等耐药,未检出耐碳青霉烯类抗生素的志贺菌。结论宁波市志贺菌流行株的血清型正在变迁中,形成F2a血清型、Fc亚型和D群志贺菌新格局。菌株耐药严重,多为ESBLs菌株,携带多种毒力基因,有较强的致病力,应引起关注。
Objective To understand the changes of serogroups, drug resistance and virulence of Shigella in Ningbo and to provide experimental evidence for the prevention and control of bacillary dysentery. Methods Strains were isolated from patients with diarrhea. API biochemical identification was performed. Serological typing was performed using slide agglutination method. VITEK 2 Compact GPS-16 card and K-B method were used for drug susceptibility. Five virulence genes were detected by PCR. Results Among 254 strains of Shigella, 170 strains (66.93%) of Shigella flexneri (group B), 83 strains (32.68%) of Shigella sonnei (group D) ) 1 strain (0.39%), the biochemical results are typical and ATCC standard strains. Virulence genes of ipa H, set1A, set1B, sen, ial and vir A were 100.00%, 80.71%, 75.20%, 85.04%, 94.49% and 88.98% respectively. Quinolones such as nalidixic acid, tetracycline and ampicillin of penicillins were found in the top three of drug resistance rate. Some strains were resistant to ceftazidime in the third generation cephalosporins, cefepime in the fourth generation cephalosporins, etc. Resistance, no detection of carbapenem-resistant Shigella antibiotics. Conclusion The serotypes of Shigella strains in Ningbo are changing, and a new pattern of Shigella spp. F2a serotype, Fc subtype and D group is formed. Strain resistant to serious, mostly ESBLs strains, carrying a variety of virulence genes, have a strong virulence, should cause concern.