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我站自79年起应用反相血疑检测疫区内鼠洞土壤的鼠疫抗原获得阳性结果。试剂系由云南省流行病防治研究所供给,反相血疑按常规方法进行,结果于1980年4月从28份土壤标本中获得4份阳性,血疑效价1:40—1:80~(++)之间,阳性检出率达14.3％。我们的体会是选取土样时应特别仔细,切勿将其它碎土落入其中。方法是在挖掘鼠洞时要边挖边查看,细心将鼠巢全部暴露(切勿将碎土落入巢内),戴防护手套轻轻将鼠巢取出装入布袋,然后把鼠巢底部表层约厚1厘米的土层取50—100克也装入同一布袋并注明编号。带回实验室除去巢穴基质及鼠粪后取样备试。
I have been stationed since 79 years of anti-phase blood test detection of plague in mice plague soil antigen positive results. The reagent was supplied by Yunnan Institute of Epidemiology and the reverse phase was conducted according to the conventional method. As a result, 4 samples were positive from 28 soil samples in April 1980, the titer of serum was 1: 40-1: 80 ~ (++), the positive detection rate of 14.3%. Our experience is that we should take special care when choosing soil samples, and never drop any other ground debris into it. The method is to excavate the rat hole while digging edge to see, carefully all exposed rat nests (not to fall into the nest of broken soil), wearing protective gloves gently remove the rat’s nest into the bag, and then the bottom surface of the nest About 1 cm thick soil to take 50-100 grams are also loaded into the same bag and indicate the number. Back to the laboratory to remove the nest matrix and mouse feces sampling preparation.