论文部分内容阅读
[目的]探讨胃癌组织中缺氧诱导因子-1α(HIF-1α)的表达及其与血管内皮生长因子(VEGF)的关系。[方法]用免疫组织化学SP法检测80例胃癌中HIF-1α、VEGF的表达,并与30例胃癌旁不典型增生组织、30例胃正常组织作为对照;分析HIF-1α、VEGF与胃癌临床病理因素及预后的关系。[结果]HIF-1α和VEGF在胃癌中的阳性表达分别为47.5%(38/80)和71.3%(57/80),明显高于胃癌旁不典型组织及胃正常组织(P<0.05)。HIF-1α、VEGF与胃癌的TNM分期以及肿瘤浸润深度、淋巴结转移等相关(P<0.05),与组织学分级无关。胃癌中HIF-1α与VEGF的表达呈显著性正相关(r=0.328,P<0.01)。[结论]HIF-1α、VEGF均在胃癌中过表达并且与胃癌的侵袭、转移及预后密切相关,HIF-1α可能通过上调VEGF的表达,在胃癌的发生、发展中发挥重要作用。
[Objective] To investigate the expression of hypoxia-inducible factor-1α (HIF-1α) and its relationship with vascular endothelial growth factor (VEGF) in gastric cancer. [Methods] The expression of HIF-1α and VEGF in 80 cases of gastric cancer was detected by immunohistochemical SP method. Compared with 30 cases of gastric atypical hyperplasia and 30 cases of gastric normal tissue, HIF-1α, VEGF and gastric cancer Relationship between pathological factors and prognosis. [Results] The positive rates of HIF-1α and VEGF in gastric cancer were 47.5% (38/80) and 71.3% (57/80), respectively, which were significantly higher than those in gastric atypical and gastric normal tissues (P <0.05). HIF-1α and VEGF were correlated with TNM staging, tumor invasion depth and lymph node metastasis (P <0.05), but not with histological grade. There was a significant positive correlation between the expression of HIF-1α and VEGF in gastric cancer (r = 0.328, P <0.01). [Conclusion] Both HIF-1α and VEGF are overexpressed in gastric cancer and closely related to the invasion, metastasis and prognosis of gastric cancer. HIF-1α may play an important role in the development and progression of gastric cancer by up-regulating the expression of VEGF.