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本文报告了TPA对32例不同类型白血病细胞的体外分化诱导结果。TPA(1.6×10~-7M)可诱导急性非淋巴细胞(ANLL)白血病细胞迅速出现单核巨噬细胞分化标志:细胞贴壁、胞浆丝状伪足形成,具有类似巨噬细胞的形态改变及相应的细胞化学反应特征。急性淋巴细胞白血病(ALL)和桨细胞白血病(PCL)细胞不发生上述变化,表现为细胞聚集成闭现象。慢性淋巴细胞白血病(CLL)出现桨细胞样形态转化。初发与复发病例的诱导反应相类似。TPA体外诱导分化实验,有助于了解病人白血病细胞的分化潜能,对于鉴别粒单系和淋巴系两类白血病,尤其对于用常规方法分型困难的低分化白血病有一定的临床诊断意义。
This article reports the results of in vitro differentiation of 32 different types of leukemia cells induced by TPA. TPA (1.6 × 10 -7 M) induces rapid monocyte-macrophage differentiation markers in acute non-lymphoid (ANLL) leukemia cells: adherent cells and cytoplasmic filopodia with morphological changes similar to macrophages And the corresponding characteristics of cytochemical reaction. Acute lymphoblastic leukemia (ALL) and paddle cell leukemia (PCL) cells do not occur in these changes, the performance of cell aggregation into a closed phenomenon. Chronic lymphocytic leukemia (CLL) appears paddle cell-like morphological transformation. Induction of initial and recurrent cases was similar. TPA in vitro differentiation experiment helps to understand the differentiation potential of patients with leukemia cells, for the identification of two kinds of single and lymphoid leukemia, especially for the poor classification of conventional methods of poorly differentiated leukemia have some clinical diagnostic significance.