论文部分内容阅读
目的:探索不同生长素类调节剂对滇杠柳扦插繁殖的影响,以及其生根过程中内源激素、氧化酶活性的动态变化规律。方法:采用不同浓度的吲哚丁酸(IBA)、生根粉1号(ABT1)和萘乙酸(NAA)处理滇杠柳插穗,检测生根效果及生根过程中内源激素吲哚乙酸(IAA)、玉米素核苷类(ZR s)、脱落酸(ABA)含量及吲哚乙酸氧化酶(IAAO)、多酚氧化酶(PPO)、过氧化物酶(POD)活性的变化。结果:150 mg/L IBA处理滇杠柳插穗生根率达到80%,150 mg/L ABT1和NAA处理的插穗生根率为70%和68%,而对照只有23%生根。滇杠柳扦插生根过程可分为不定根诱导期、露白期和生长期3个阶段。不定根诱导期需要低含量的内源激素IAA、ZR s、ABA、较高活性的IAAO;不定根形成露白期需要高含量内源激素IAA、较高活性的PPO、低含量的ZR s、ABA和低活性的IAAO和POD;不定根生长期需要一定含量的IAA、ZR s、ABA和较高活性的PPO、POD、IAAO,在生根后期三种氧化酶活性下降。生根过程中,150 mg/L IBA的处理增加了插穗内IAA含量和PPO活性,降低了插穗内ABA、ZR s含量和IAAO、POD活性。结论:内源IAA、ZR s、ABA的含量和IAAO、PPO、POD活性的动态变化与插穗不定根生长过程密切相关。
OBJECTIVE: To explore the effects of different auxin regulators on the cuttage propagation of D. yunnanensis as well as the dynamic changes of endogenous hormones and oxidase activities during rooting. Methods: Different concentrations of IBA, ABT1 and NAA were used to treat cuttings of D. yunnanensis, and the effects of rooting and endogenous hormones IAA, ZR s, ABA and the changes of IAAO, PPO and POD activities. Results: The rooting rate of cuttings of P. orientalis reached to 80% with 150 mg / L IBA. The rooting rates of cuttings with 150 mg / L ABT1 and NAA treatments were 70% and 68%, while only 23% of the control roots. DianBaowu cuttings rooting process can be divided into adventitious root induction period, white stage and the growth of three phases. Adventitious root induction required low levels of endogenous hormones IAA, ZR s, ABA, higher activity of IAAO; adventitious root formation of exposed white need high endogenous hormone IAA, higher activity of PPO, low levels of ZR s, ABA and low Active IAAO and POD. Adventitious root growth required certain levels of IAA, ZR s and ABA and higher activities of PPO, POD and IAAO. The activities of three oxidases decreased during the later rooting period. In the process of rooting, IAA and PPO activities in cuttings were increased by 150 mg / L IBA treatment, and ABA and ZR s contents and IAAO and POD activities in cuttings were decreased. Conclusion: The content of IAA, ZR s, ABA and the dynamic changes of IAAO, PPO and POD activities are closely related to adventitious root growth process.