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将单个细胞涂片连续进行~3HTdR-放射自显术和福尔根反应,先计算银粒标记的增殖细胞(S,G_2)的比例,再用细胞光度法测量未标记细胞(GI)的福尔根光密度。虽然受PHA刺激的活性淋巴细胞的标记指数达30.17％,但幼年性息肉不能参入同位素,各种腺瘤性息肉和腺瘤中只有少数标记细胞。以前中期(4C)淋巴细胞的相对DNA含量(24.06±2.67)为衡量标准,在与二倍体值(2C,12.03)比较后算出测量细胞的DI。5例幼年性息肉细胞的DNA含量为二倍体值(平均12.06,DT=1.00),5例腺瘤性息肉和腺瘤为超二倍体值(平均14.36,DI=1.19)。两类息肉的DNA含量差异显著。鉴于前者与腺癌无关,后者易于癌变。因此,不正常DNA含量或非整倍体性可能是细胞癌变的征兆。
Single cell smears were serially subjected to ~3HTdR-radioautocognition and Folgogen responses. The proportion of silver-labeled proliferating cells (S, G 2 ) was first calculated, and the unlabeled cells (GI) were measured by cytometry. Ergen optical density. Although the labeling index of active lymphocytes stimulated by PHA is 30.17%, juvenile polyps do not participate in isotopes. There are only a few labeled cells in various adenomatous polyps and adenomas. The relative DNA content of the previously intermediate (4C) lymphocytes (24.06±2.67) was used as a measure, and the DI of the measured cells was calculated after comparing with the diploid value (2C, 12.03). The DNA content of 5 juvenile polyp cells was diploid (mean 12.06, DT = 1.00), and the adenomatous polyps and adenomas were hyperdiploid in 5 cases (mean 14.36, DI = 1.19). The DNA content of the two types of polyps differs significantly. Whereas the former is not associated with adenocarcinoma, the latter is prone to canceration. Therefore, abnormal DNA content or aneuploidy may be a sign of cancerous cells.