将烟草叶片经过固定、水解、剥离、降解、浸泡、置换、干燥、喷金等处理 ,成功地制备出了烟草叶片各层组织的扫描电镜样品 ;首次获得烟草叶片各组织层的立体结构图像 ,通过比较 ,发现不同部位叶片内部组织结构存在某些规律差异。为研究烟草叶片各组织层中细胞分布和生长发育规律提供了一条新的途径。 The tobacco leaf samples were successfully prepared by scanning electron microscopy (SEM), which were fixed, hydrolyzed, exfoliated, degraded, soaked, replaced, dried and sprayed with gold. The three- By comparison, we found that there are some regular differences in the internal structure of leaves in different parts. It provided a new way to study the distribution of cells and the growth and development rules of tobacco leaf tissue layers.