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近年许多学者相继报道人类和动物胎盘中T_4内环脱碘酶的活性。并且指出人胎盘中由T_4产生的rTs浓度随妊娠时间而明显改变。这些改变也影响羊水中rT_3浓度。作者测定了不同妊娠时间妇女胎盘中T_4、T_3和rT_3浓度,并与母体和脐带血清中的甲状腺激素浓度进行比较。 实验需胎盘分别取自妊娠20—23周流产者(7例),妊娠24—26周早产者(9例),正常分娩者(16例)和突眼性甲状腺肿(Graves)病的妊娠者(4例)。胎盘取出后,经匀浆、离心,用99％乙醇提取T_4、T_3和rT_3,并用放射免疫法进行测定。
In recent years, many scholars have reported human and animal placenta T_4 endocyclic deiodinase activity. And pointed out that the concentration of rTs produced by T_4 in the human placenta significantly changes with time of gestation. These changes also affect the rT_3 concentration in amniotic fluid. The authors measured the levels of T_4, T_3 and rT_3 in the placenta of women of different gestational ages and compared them with the concentrations of thyroid hormone in the maternal and cord serum. The experimental placenta were taken from 20-23 weeks of pregnancy, abortion (7 cases), 24-26 weeks of pregnancy, premature birth (9 cases), normal delivery (16 cases) and Graves’ disease of pregnancy (4 cases). After removal of the placenta, homogenate and centrifuge, T_4, T_3 and rT_3 were extracted with 99% ethanol and determined by radioimmunoassay.