番茄花叶病毒(ToMV)的弱毒疫苗,在京郊春保护地番茄试用四年来,由于能够抗病,增产和提高产值,因此深受农民欢迎。不过,使用方法不够简便,又常常造成死苗现象,以致于推广起来有一定困难。迄今为止,我们试用过三种接种方法,即磨擦法,浸根法和喷射法。磨擦法,是植物病毒学试验的常规接种方法,具有感染率高,操作简便等优点,但是工作效率低,生产上难以 ToMV attenuated vaccine, in the suburbs of Beijing in the spring of protective tomato trial four years, due to be able to disease resistance, increase production and increase output value, so welcomed by farmers. However, the method of use is not simple enough and often results in the phenomenon of dead seedling, which makes it difficult to promote it. So far, we have tried three methods of inoculation, namely friction method, dip root method and injection method. Friction method, is a routine virulence test of plant inoculation method, with a high infection rate, easy operation, etc., but the work efficiency is low, difficult to produce