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目的探讨钙、镁、铜、锌、维生素D和大豆联合干预对燃煤型氟中毒雌鼠氟斑牙、骨氟和尿氟的影响。方法将120只SD雌鼠分为对照组、染氟组、高氟+钙尔奇牌添佳片组(即多种元素干预组)、高氟+钙尔奇牌添佳片+大豆组(即多种元素+大豆干预组),每组30只,动物自由食用不同配方的织金病区原煤加拌泥煤烘烤的玉米饲料,构建燃煤型氟中毒动物模型。每组分别于染氟60、120、180 d选择10只大鼠,动态观察雌鼠染氟期间氟斑牙情况,分别采用离子选择电极法、高温灰化-氟离子选择电极法测定雌鼠尿氟、骨氟含量。结果随着染氟时间的延长,氟斑牙症状加重,干预组大鼠氟斑牙程度比染氟组轻。染氟组雌鼠尿氟、骨氟含量均高于同一时间点(60、120、180 d)的对照组,差异有统计学意义(P<0.05)。同一时间点时,染氟组、多种元素干预组、多种元素+大豆干预组雌鼠的尿氟含量依次增加,差异均有统计学意义(P<0.05);染氟组雌鼠骨氟含量高于对照组,但多种元素干预组、多种元素+大豆干预组骨氟含量均低于染氟组,差异有统计学意义(P<0.05)。随着染毒时间的延长,染氟组、多种元素干预组、多种元素+大豆干预组的尿氟含量均逐渐增加,差异有统计学意义(P<0.05);染氟组骨氟含量逐渐增加,多种元素干预组、多种元素+大豆干预组骨氟含量逐渐降低,差异均有统计学意义(P<0.05)。结论高剂量氟对雌鼠有明显毒性作用,多种营养素干预可拮抗其毒性作用,若在此基础上添加大豆联合干预则拮抗作用更明显。
Objective To investigate the effects of combined intervention of calcium, magnesium, copper, zinc, vitamin D and soybean on dental fluorosis, bone fluoride and urinary fluoride in coal-type fluorosis women. Methods 120 female SD rats were divided into control group, high fluoride group, high fluoride group, high calcium group, That is, a variety of elements + soybean intervention group), each group of 30 animals free to eat different formulations of Zhijin ward raw coal mixed with peat bake corn feed to build coal-fired fluorosis animal model. Ten rats in each group were dyed with fluorine for 60,120 and 180 days, respectively. The dynamic changes of dental fluorosis in female rats during fluoridation were observed. The levels of urinary excretion were determined by ion-selective electrode method and high-temperature ashing-fluoride ion selective electrode method Fluoride, bone fluorine content. Results With the extension of fluoridation time, the symptoms of dental fluorosis increased, and the dental fluorosis in intervention group was lighter than that in fluorosis group. Urinary fluorosis and bone fluoride levels in female rats exposed to fluoride were significantly higher than those in the control group at the same time point (60, 120 and 180 days) (P <0.05). At the same time point, the levels of urinary fluoride in the fluoride-exposed group, multiple-element intervention group and multiple-element + soybean intervention groups increased in turn, with statistical significance (P <0.05) But the content of bone fluoride in multi-element intervention group and multi-element + soybean intervention group was lower than that in fluoride group, the difference was statistically significant (P <0.05). With the prolongation of exposure time, the contents of urinary fluoride in fluoride-treated group, multi-element-treated group and multi-element + soybean intervention group increased gradually, with significant difference (P <0.05) (P <0.05). The content of bone fluoride in multi-element intervention group and multi-element + soybean intervention group gradually decreased. The difference was statistically significant (P <0.05). Conclusion High dose of fluoride has significant toxic effect on female rats. The intervention of multiple nutrients can antagonize the toxic effect. If combined with soybean, the antagonism is more obvious.