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目的：提取伤寒沙门氏菌ＳＯＤ，研究ＳＯＤ的抗原性及为进一步研究ＳＯＤ与细菌毒力的关系提供材料。方法：经硫酸铵分级沉淀，ＤＥＡＥ纤维素柱层析提取伤寒沙门氏菌ＳＯＤ。用聚丙烯酰胺凝胶电泳，蛋白质染色及酶活性染色鉴别ＳＯＤ纯度。采用原子吸收分光光度法和酶活性抑制试验检测ＳＯＤ的辅基。将提取的ＳＯＤ免疫家兔制备兔抗ＳＯＤ血清，用双向琼脂扩散试验和抗体对酶活性抑制试验分析伤寒沙门氏菌ＳＯＤ的抗原性。结果：所提取的ＳＯＤ比活性为３２７０Ｕ／ｍｇ，其纯度达到了电泳纯，辅基为Ｆｅ原子。双向琼脂扩散试验结果显示抗伤寒沙门氏菌Ｆｅ－ＳＯＤ血清与牛红细胞ＳＯＤ不形成沉淀线，抗血清可抑制伤寒沙门氏菌及鼠伤寒沙门氏菌Ｆｅ－ＳＯＤ和Ｆｅ／Ｍｎ－ＳＯＤ活性，对Ｍｎ－ＳＯＤ活性无抑制作用。结论：伤寒沙门氏菌Ｆｅ－ＳＯＤ与牛红细胞ＳＯＤ无交叉反应，但和鼠伤寒沙门氏菌ＳＯＤ之间有共同抗原，也提示ＳＯＤ的辅基似乎决定了其抗原特异性
OBJECTIVE: To extract SOD of S. typhi, to study the antigenicity of SOD and to provide material for further study on the relationship between SOD and bacterial virulence. Methods: The SOD of Salmonella typhi was extracted by ammonium sulfate fractionation and DEAE cellulose column chromatography. The purity of SOD was identified by polyacrylamide gel electrophoresis, protein staining and enzyme activity staining. Apoptosis was detected by atomic absorption spectrophotometry and enzyme activity inhibition assay. Rabbit anti-SOD serum was prepared by immunizing rabbits with extracted SOD, and the antigenicity of SOD of Salmonella typhi was analyzed by two-way agar diffusion test and antibody inhibition test. Results: The specific activity of SOD extracted was 3270U / mg, its purity reached electrophoretic purity, prosthetic group was Fe atom. Two-dimensional agar diffusion test results showed that the anti-Salmonella Fe-SOD serum and bovine red blood cell SOD did not form a precipitation line, the antiserum inhibited Salmonella typhimurium and Salmonella typhimurium Fe-SOD and Fe / Mn-SOD activity, Mn-SOD activity was not inhibited effect. CONCLUSIONS: Salmonella typhimurium Fe-SOD does not cross-react with bovine red blood cell SOD but has a common antigen with Salmonella typhimurium SOD, suggesting that the cofactor of SOD appears to determine its antigen specificity