目的研究庚型肝炎病毒（ＨＧＶ）在恒河猴中的实验感染状态。方法用一名ＨＧＶＲＮＡ阳性、ＨＢＶ、ＨＣＶ均阴性的健康献血员血浆实验感染２只恒河猴，并取第一代猴感染后６周的血再感染１只第二代恒河猴，然后用以第二代猴感染６周后血继续感染２只第三代恒河猴。分别用逆转录聚合酶链反应（ＲＴ－ｎＰＣＲ）检测受感染猴血清中的ＨＧＶＲＮＡ，并每周抽血测定血清中丙氨酸转氨酶（ＡＬＴ）。结果感染１周后猴血清ＨＧＶＲＮＡ阳转，最长持续阳性２８周以上。不同感染个体血清ＡＬＴ水平有明显差异，其中１号猴有短期轻度升高，５号猴血清ＡＬＴ较长时间在１００Ｕ／Ｌ以上。肝活检发现，感染后１６周猴肝组织出现明显的病毒性肝炎样病理改变。进一步对该献血员血浆和感染后猴血清中的ＨＧＶ５’端部分非编码区基因ＰＣＲ产物进行测序，结果显示感染用献血员血浆和猴血清中ＨＧＶ序列与国外株ＨＧＵ４４４０２的同源性分别为９８３３％和９５８３％；与ＨＧＵ３６３８０株的同源性分别为９２５０％和８９１７％；感染猴血清中ＨＧＶ序列与献血员ＨＧＶ序列同源性为９５８３％。结论恒河猴对ＨＧＶ敏感，可以做为实验模型动物 Objective To investigate the experimental infection status of hepatitis G virus (HGV) in rhesus monkeys. Methods Two Hamster monkeys were infected with one HGV RNA-positive, negative HBV and HCV-negative healthy blood donor. One second-generation rhesus monkeys were infected with the second-generation rhesus monkeys after 6 weeks of infection. The second generation of monkeys infected with 6 weeks after the blood continued to infect 2 third-generation rhesus monkeys. HGV RNA in sera of infected monkeys was detected by reverse transcription polymerase chain reaction (RT-nPCR), and serum alanine aminotransferase (ALT) was measured weekly. Results After 1 week of infection, the monkey serum HGVRNA positive rotation, the longest sustained positive for more than 28 weeks. Serum ALT levels of different infected individuals were significantly different, in which monkey No. 1 had a slight increase in the short term, and monkey 5 serum ALT longer than 100U / L. Liver biopsy revealed significant pathological changes of viral hepatitis in monkey liver tissues 16 weeks after infection. The PCR products of the non-coding region of HGV 5 ’end of the plasma and post-infection monkey serum were further sequenced. The results showed that the homology of the HGV sequence in the plasma and monkey serum of foreign donors with that of foreign strain HGU44402 was 98  33% and 9583%, respectively. The homologies with HGU36380 strains were 9250% and 8917% respectively. The homology of the HGV sequences in blood serum of infected monkeys with that of HGV was 9583%. Conclusion Rhesus monkey is sensitive to HGV and can be used as experimental animal model