本试验用油桐尺蠖核型乡角体病毒(Buzura Suppressaria Nuclear Polyhedro-sis Virus)感染油桐尺蠖卵巢细胞系,分别置于0℃、15℃、20℃、26℃、28℃、30℃、37℃下静止培养,观察细胞病理变化,测定细胞的感染百分率和多角体含量。试验结果表明,不同温度对病毒的感染率及其在细胞中复制有明显的影响。26℃是油桐尺蠖核型多角体病毒感染卵巢细胞并形成多角体的最适培养温度,感染率最高,多角体含量也最多。培养温度过低或过高,多角体都不能在细胞内复制。 In this study, the ovules of Taraxacum mongolicum were infected with Buzura Suppressaria Nuclear Polyhedro-sis Virus and were exposed to 0, 15, 20, 26, 28, 30, 37 ℃ stationary culture, observed cell pathological changes, the percentage of cells infected and the determination of polyhedrosis content. The test results show that different temperatures have a significant impact on the virus infection and its replication in cells. 26 ℃ is the optimum temperature for infection of ovary cells and formation of polyhedrosis by TULF nuclear karyopyr virus, with the highest infection rate and the highest polyhedrosis content. Incubation temperature is too low or too high, polyhedrosis can not be copied in the cell.