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为了解草莓与恶疫霉[Phytophthora cactorum(Lebert&Cohn)J.Schrt]互作的分子机制,以感病草莓品种FDP821为材料,以接种恶疫霉的草莓冠部为处理、未接种健康生长的草莓冠部为对照,利用抑制性差减杂交技术构建恶疫霉侵染诱导的草莓差异表达的cDNA差减文库。PCR鉴定随机挑取的阳性克隆,显示插入片段大小为300～1 000bp。随机挑取109个克隆进行测序,获得92个草莓EST,分析表明来自50个草莓基因。BLASTX比对结果表明:这些草莓基因的功能涉及抗病防卫机制、抗氧化作用、物质代谢和蛋白质合成等。这为理解草莓冠腐病抗性分子机制奠定了重要的数据基础。
In order to understand the molecular mechanism of the interaction between strawberry and Phytophthora cactorum (Lebert & Cohn) J.Schrt, the susceptible strawberry variety FDP821 was treated with Phytophthora capsici-treated strawberry crown and uninoculated with healthy growth Strawberry crown as a control, the suppression subtractive hybridization technique was used to construct a cDNA subtractive library of differentially expressed strawberry induced by Phytophthora infestans. PCR identification of positive clones picked randomly showed that the insert size of 300 ~ 1 000bp. A total of 109 clones were randomly selected and sequenced to obtain 92 strawberry ESTs, which were analyzed from 50 strawberry genes. The result of BLASTX alignment showed that the function of these strawberry genes involved in disease resistance and defense mechanisms, antioxidation, metabolism and protein synthesis. This laid an important data foundation for understanding the molecular mechanism of strawberry crown rot resistance.