论文部分内容阅读
目的建立RP-HPLC双波长法同时测定活络效灵丹中3种丹参酮和6种乳香酸的含量。方法采用RP-HPLC法,色谱柱为Waters x BridgeTmC18柱(150 mm×4.6 mm,3.5μm),流动相为体积分数为0.01%的磷酸水溶液-乙腈,梯度洗脱,流速为1.0 m L·min-1,检测波长为210 nm和254 nm,柱温为30℃。结果隐丹参酮、丹参酮I、丹参酮IIA、11-羰基-β-乳香酸(KBA)、11-羰基-β-乙酰乳香酸(AKBA)、α-乳香酸(α-BA)、β-乳香酸(β-BA)、乙酰基-α-乳香酸(A-α-BA)和乙酰基-β-乳香酸(A-β-BA)的质量浓度分别在0.252 0~8.064、0.157 2~5.030、0.937 5~30.00、3.156~101.0、25.10~803.2、6.250~200.0、6.813~218.0、13.50~432.0、12.50~400.0 mg·L-1内与峰面积呈良好的线性关系(r≥0.999 3,n=6),提取回收率均在97.2%~101.2%内。结论该方法可做为活络效灵丹中丹参酮类和乳香酸类化学成分的含量测定方法,为其质量控制提供参考依据。
OBJECTIVE To establish a RP-HPLC dual-wavelength method for simultaneous determination of three tanshinones and six boswellic acids in Huoxinglingling. Methods The RP-HPLC method was used. The column was a Waters x BridgeTmC18 column (150 mm × 4.6 mm, 3.5 μm). The mobile phase consisted of a 0.01% phosphoric acid solution in acetonitrile with a gradient elution at a flow rate of 1.0 mL · min -1, the detection wavelength was 210 nm and 254 nm, the column temperature was 30 ℃. Results The contents of cryptotanshinone, tanshinone I, tanshinone IIA, 11-carbonyl-β-boswellic acid (KBA), 11-carbonyl- β -acetylboswellic acid (AKBA), α-boswellic acid β-BA, A-α-BA and A-β-BA were respectively in the range of 0.252 0 ~ 8.064,0.157 2 ~ 5.030,0.937 5 ~ 30.00,3.156 ~ 101.0,25.10 ~ 803.2,6.250 ~ 200.0,6.813 ~ 218.0,13.50 ~ 432.0,12.50 ~ 400.0 mg · L-1 showed a good linear relationship with the peak area (r≥0.999 3, n = 6 ), Extraction recovery rates were within 97.2% ~ 101.2%. Conclusion This method can be used as a method for determination of the contents of tanshinones and boswellic acids in Active Rhubarb, and provide a reference for quality control.