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目的 建立复方丹参方中丹参脂溶性成分的含量测定方法 ,初步探讨丹参与三七配伍后的变化规律。方法 采用HPLC法 ,以甲醇—水 (75∶2 5 )为流动相 ,于 2 5 4nm处检测。结果 丹参脂溶性成分的含量高低依次是隐丹参酮、丹参酮ⅡA、丹参酮Ⅰ ;复方丹参方中丹参 /三七配比不同时 ,成分的含量及溶液稳定性有所差异。结论 本法简便、快速、准确 ,可作为复方丹参方的质量控制标准 ;复方丹参方中丹参脂溶性成分的稳定性及含量与丹参 /三七的配比存在非线性关系
Objective To establish a method for determining the content of lipid-soluble constituents of Danshen in prescription Danshenfang, and to explore the changing regularity of Dan’s participation in Panax notoginseng compatibility. Methods HPLC method was used. The mobile phase was methanol-water (75∶25) and detected at 254 nm. Results The content of fat-soluble components in Danshen was successively cryptotanshinone, tanshinone IIA, and tanshinone I. When the ratio of compound Danshen to Panax notoginseng was different in compound Danshen formula, the composition and solution stability were different. Conclusion This method is simple, rapid and accurate, and can be used as a quality control standard for compound Danshen prescription. The stability and content of lipophilic components of Salvia miltiorrhiza in compound Danshen formula has a nonlinear relationship with the ratio of Salvia miltiorrhizae/Panax notoginseng.