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目的:观察细胞角质素19(CK19)及消减基因P02在肝细胞癌,肝硬化组织及卵圆细胞中的表达情况.方法:采用免疫组化SP法观察8例正常肝组织,27例肝硬化组织和43例肝细胞癌组织中CK19的表达.采用DIG-probe synthesis kit,聚合酶链反应方法制备P02探针,通过原位杂交方法检测P02在肝细胞癌,肝硬化组织及卵圆细胞中的表达.结果:CK19在肝硬化组与正常肝组织之间表达率无明显差异,但在肝细胞癌组与肝硬化组之间差异有显著性(69.77% vs 25.93%,P<0.01).肝硬化组织与肝细胞癌组织中CK19标记的卵圆细胞数量有显著性差异(5.74±1.05 vs 10.51±1.78,P<0.01).P02在肝硬化和肝细胞癌中的阳性表达率分别为26.7%和80%,二者之间表达有显著性差异(P<0.01).结论:CK19可能参与了肝硬化到肝细胞癌的癌变过程.卵圆细胞与肝脏损伤后再生及癌变有关,可能是P02通过促进卵圆细胞的增殖来介导肝细胞癌的发生.
Objective: To observe the expression of CK19 and P02 in hepatocellular carcinoma, cirrhosis and oval cells.Methods: 8 cases of normal liver tissue and 27 cases of liver cirrhosis were observed by immunohistochemistry And the expression of CK19 in 43 cases of hepatocellular carcinoma.P02 probe was prepared by polymerase chain reaction using the DIG-probe synthesis kit and P02 was detected by in situ hybridization in hepatocellular carcinoma, cirrhosis and oval cells The expression of CK19 was not significantly different between cirrhosis and normal liver tissues, but there was a significant difference between hepatocellular carcinoma and cirrhosis (69.77% vs 25.93%, P <0.01). The number of CK19-labeled oval cells in cirrhotic and hepatocellular carcinoma tissues was significantly different (5.74 ± 1.05 vs 10.51 ± 1.78, P <0.01) .The positive expression rates of P02 in cirrhosis and hepatocellular carcinoma were 26.7 % And 80%, respectively (P <0.01) .Conclusion: CK19 may be involved in the carcinogenesis of hepatocellular carcinoma from cirrhosis.Oval cells may be related to the regeneration and carcinogenesis after liver injury, which may be P02 mediates hepatocellular carcinoma by promoting oval cell proliferation.