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植物名称:桔梗(Platycodon grandiflorum)。材料类别:叶片。选两年生桔梗植株,在花蕾形成前后分批采摘叶片,均用饱和漂白精片水溶液消毒20分钟,无菌水冲洗2—3次,分别接种。培养条件。以MS为基本培养基,添加水解乳蛋白500mg/l,将 NAA0.5ppm和 6-BA1ppm配比组成培养基Ⅰ,MS+2,4-D 0.2ppm组成培养基Ⅱ,培养基Ⅲ为1/2 MS+IBA 0.5mg/l。材料接种后,先在黑暗或微光下培养25—30天,再移到光照度2000lx的条件下培养,培养温度为20—28℃。
Plant name: Campanulaceae (Platycodon grandiflorum). Material Category: Leaves. Two-year-old Campanulaceae plants were selected before and after the formation of buds were picked leaves in batches, are saturated bleach fine pieces of aqueous solution disinfection 20 minutes, sterile water rinse 2-3 times, respectively, inoculation. Culture conditions. MS medium supplemented with hydrolyzed milk protein 500mg / l, NAA0.5ppm and 6-BA1ppm formulated medium Ⅰ, MS + 2,4-D 0.2ppm composed of medium Ⅱ, medium Ⅲ is 1 / 2 MS + IBA 0.5 mg / l. After the material inoculation, the first 25-30 days in the dark or low light culture, and then moved to light illumination under the conditions of 2000lx culture, culture temperature is 20-28 ℃.