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为探讨花生耐低温的遗传机理,实现耐低温分子标记辅助选择育种的长远目标,并培育耐低温的高油酸品种,搭配了2个普通油酸×高油酸杂交组合。首先通过近红外技术初筛,选择油酸含量预测值高于40%的F1单粒种子,进一步经转座子标记鉴定,分别获得了32粒和24粒真杂种。两对转座子标记引物Ah TE0398和Ah TE0167鉴定结果完全一致,也与FAD2等位基因特异PCR(AS-PCR)结果一致,证实了转座子标记鉴定技术的可靠性。
In order to explore the genetic mechanism of low temperature tolerance in peanut and to achieve the long-term goal of breeding low-temperature resistant molecular marker-assisted breeding, and to cultivate a high oleic acid variety with low temperature tolerance, two common combinations of oleic acid × high oleic acid were used. Firstly, the F1 single-seed with the predicted value of oleic acid higher than 40% was selected by near infrared spectroscopy and further identified by the transposon marker, and 32 and 24 true hybrids were obtained respectively. The results of the two pairs of transposon markers Ah TE0398 and Ah TE0167 were identical and consistent with the FAD2 allele specific PCR (AS-PCR) results, confirming the reliability of transposon marker identification technology.