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目的:研究甘肃栽培党参的亲缘关系、居群结构及遗传多样性分析。方法:采用SSR分子标记技术对供试材料进行标记,利用POPGEN和NTSYS软件对数据进行整理分析。结果:筛选出16对引物,共扩增出125个条带,多态性条带120个,多态位点百分率(PPB)为96%。平均等位基因数(Na)1.9524,平均有效等位基因数(Ne)1.5078,平均Nei’s基因多样性(H)0.3061,平均多态性信息含量(PIC)0.3378,平均Shannon’s信息指数(I)0.4658。6个党参居群的总遗传多样度(Ht)为0.3118,居群内遗传多样度(Hs)为0.2655,居群间遗传分化系数(G_(st))为0.1484,基因流(N_m)为2.8685。结论:甘肃党参种质资源具有丰富的遗传多样性,遗传基础较窄,遗传变异主要存在于居群内部。
Objective: To study the genetic relationship, population structure and genetic diversity of Codonopsis pilosula cultivated in Gansu Province. Methods: SSR markers were used to mark the materials tested, and the data were analyzed by POPGEN and NTSYS software. Results: Sixteen pairs of primers were screened out, of which 125 bands were amplified and 120 polymorphic bands were amplified. The percentage of polymorphic loci (PPB) was 96%. The average number of alleles (Na) 1.9524, the average number of effective alleles (Ne) 1.5078, the average Nei’s gene diversity (H) 0.3061, the average polymorphism information content (PIC) 0.3378, the average Shannon’s information index (I) 0.4658 The total genetic diversity (Ht) of 6 Codonopsis pilosula populations was 0.3118, the genetic diversity (Hs) was 0.2655, the genetic differentiation coefficient (G st) was 0.1484, the gene flow (Nm) was 2.8685. Conclusion: Codonopsis ginseng germplasm resources are rich in genetic diversity, genetic basis is narrow, genetic variation mainly exists within the population.