论文部分内容阅读
Mitochondria carry out many essential metabolic processes,the dynamic of which impacts most aspects of cellular physiology (Balk and Leaver,2001;Rose and Sheahan,2012).Therefore,characterizing the real-time metabolism of mitochondria is of great biological significance.The major challenge for reliable and authentic measurement of mitochondrial metabolites is the pure and rapid isolation of mitochondria,which can avoid the contamination from other organelles and prevent the significant loss of mitochondrial metabolites due to the transmembrane transport and the residual enzymatic reactions during isolation procedure.