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用小麦( Triticum aestivum L.) 第二部分同源群的36 个探针,对携带抗白粉病( Erysiphe graminisf.sp tritici) 基因Pm6 的普通小麦提莫菲维小麦( T.timopheevi Zhuk .) 渐渗系进行检测,通过这些渐渗系与受体亲本“Prins”之间杂交谱带多态性的比较,发现所测出多态性标记位点均位于这5 个渐渗系的2B 染色体上,但其渗入片段的位置与大小有明显区别。IGV1_456 和IGV1_458 被鉴定为2B 染色体从短臂标记Xcdo405 到长臂标记Xbcd135 之间的区域已被提莫菲维2G 染色体区段所代替;而IGV1_463 则在2B 染色体长臂Xbcd307 到Xcdo678 标记之间的区域被提莫菲维渗入成分所代替;IGV1_464 、IGV1_465 2BL染色体上的渗入片段更小,即IGV1_464 在2BL染色体上标记Xpsr934 与Xbcd135 之间的区域有提莫菲维2G 染色质成分渗入,IGV1_465 仅在Xbcd135 附近有更小的外源成分渗入。根据5个渐渗系渗入成分重叠区段比较可把Pm6 定位于2BL染色体上Xbcd135 2BL标记的邻近区域内
Thirty-six probes from the homologous group of the second part of wheat (Triticum aestivum L.) were used to amplify T. timopheevi Zhuk., A strain of Pm6 carrying resistance to powdery mildew (Erysiphe graminisp. Tritici) Introgression lines were detected by comparing the polymorphisms of the hybridization bands between these introgression lines and their parental “Prins”, and found that the polymorphic marker loci were located in the 5 introgression lines of the 2B chromosome However, the location and size of infiltration fragments are obviously different. Regions between IGV1_456 and IGV1_458 that were identified as 2B chromosomes from the short arm marker Xcdo405 to the long arm marker Xbcd135 have been replaced by the Timofovir 2G chromosome segment while IGV1_463 has been mapped between the 2B long arm Xbcd307 to Xcdo678 markers The region was replaced by Timofeevi’s infiltrating components; the infiltrative fragments on IGV1_464 and IGV1_465 2BL chromosomes were smaller, that is, IGV1_464 was infiltrated by Timofeev’s 2G chromatin component in the region between Xpsr934 and Xbcd135 on chromosome 2BL, and IGV1_465 There is a smaller influx of exogenous components around the Xbcd135. Pm6 can be located in the vicinity of the Xbcd135 2BL marker on chromosome 2BL based on the five introgression lines infiltrated into the overlapped segments