论文部分内容阅读
为了揭示动脉粥样硬化发生发展过程中血管平滑肌细胞增殖与凋亡的关系及其调节机制，采用末端脱氧核糖核酸转移酶介导的dUTP缺口末端标记（TUNEL）染色法对家兔动脉粥样硬化斑块组织中平滑肌细胞的凋亡情况进行原位检测，并对原癌基因bcl-2、c-myc和P53在斑块组织中的表达活性进行North－ernblot分析。结果发现，在动脉粥样硬化斑块组织中有许多TUNEL染色阳性的平滑肌细胞，提示斑块组织中有平滑肌细胞凋亡发生：其斑块组织的DNA电泳图谱呈梯形状．符合细胞凋亡的特征性改变。Northern印迹分析表明，在动脉粥样硬化斑块组织中，原癌基因bck-2和P53的表达较正常组织增强，C－myc表达减低。提示这些基因的表达对动脉粥样硬化斑块组织中的平滑肌细胞凋亡起着重要的调节作用。
In order to reveal the relationship between the proliferation and apoptosis of vascular smooth muscle cells and the regulatory mechanism during the development of atherosclerosis, terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) staining was used to detect the expression of atherosclerosis The apoptosis of smooth muscle cells in plaque was detected in situ, and North-ernblot analysis of the expression activity of the oncogenes bcl-2, c-myc and P53 in plaque was performed. The results showed that there are many TUNEL-positive smooth muscle cells in atherosclerotic plaque tissue, suggesting that there is smooth muscle cell apoptosis in plaque tissue: the DNA electrophoresis map of the plaque tissue has a trapezoidal shape. In line with the characteristic changes of apoptosis. Northern blot analysis showed that in atherosclerotic plaque tissues, the expressions of the oncogenes bck-2 and P53 were enhanced and the expression of C-myc was decreased. Suggesting that the expression of these genes plays an important regulatory role in the apoptosis of smooth muscle cells in atherosclerotic plaque tissue.