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目的:探讨父源性人类白细胞分化抗原HLA-A基因与子痫前期的相关性。方法:采用序列特异性引物技术(PCR-SSP)对53对子痫前期患者及其配偶和51对正常孕妇及其配偶进行HLA-A等位基因分型,比较其基因频率,并对两组夫妇HLA-A等位基因型别配伍进行比较分析。结果:子痫前期患者和正常晚孕者中检出14个HLA-A等位基因,配偶中检出13个HLA-A等位基因。子痫前期组配偶HLA-A24基因频率显著低于正常晚孕组(P<0.05);两组孕妇及其配偶的其它HLA-A基因型别无显著差异。子痫前期组夫妇均携带HLA-A11基因的频率显著高于正常晚孕组(P<0.05);子痫前期组妇不携带HLA-A24而夫携带HLA-A24基因的频率显著低于正常晚孕组(P<0.05);子痫前期组夫妇均不携带HLA-A24基因的频率显著高于正常晚孕组(P<0.05)。结论:夫妇间的某些特定HLA-A基因配伍模式与子痫前期的发病相关。
Objective: To investigate the relationship between HLA-A of paternal human leukocyte differentiation antigen and preeclampsia. Methods: HLA-A alleles were genotyped in 53 pairs of preeclampsia and their spouses and 51 pairs of normal pregnant women and their spouses by PCR-SSP. The frequencies of genes were compared between the two groups Couple HLA-A allele type compatibility for comparative analysis. Results: 14 HLA-A alleles were detected in preeclampsia and normal pregnant women, and 13 HLA-A alleles were detected in mate. The frequency of HLA-A24 gene in preeclampsia group was significantly lower than that in normal pregnancy group (P <0.05). Other HLA-A genotypes of pregnant women and their spouses had no significant difference. The frequency of HLA-A11 gene in preeclampsia group was significantly higher than that in normal pregnancy group (P <0.05). The frequency of HLA-A24 gene in non-preeclampsia group was significantly lower than that in normal preeclampsia group Pregnant group (P <0.05). The frequency of HLA-A24 gene expression in the preeclampsia group was significantly higher than that in the normal pregnancy group (P <0.05). CONCLUSIONS: Some patterns of HLA-A compatibility between couples are associated with the development of preeclampsia.