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为了解来自广东和广西的瓜类疫霉的遗传多样性,利用从180条RAPD引物中所筛选出的多态扩增性强、重复性好的12条引物,对分离自两省区的96株瓜类疫霉进行了全基因组DNA遗传多样性分析和指纹图谱构建。通过对供试菌株的RAPD-PCR扩增,共获得135条DNA标记谱带,其中124条为多态性谱带,多态检测率为91.9%。利用NTSYSpc Version2.1软件对供试菌株间的遗传距离进行聚类分析并构建系统树,以遗传相似系数0.81为阈值,将96个供试菌株划分为12个RAPD群,多数分离物之间遗传相似性较低,在DNA水平上存在显著的遗传变异,具有较丰富的遗传多样性。不同地区间菌株的遗传分化程度不同,分离自黄瓜的菌株遗传分化明显高于分离自冬瓜的菌株。RAPD群与菌株地理来源、分离寄主、致病力、交配型及甲霜灵抗性均无明显的相关性。
In order to understand the genetic diversity of P. melon from Guangdong and Guangxi, 12 primers with strong repeatability and good repeatability were selected from 180 RAPD primers. Phylogenetic analysis of strain Phytophthora gingivalis showed that the genetic diversity of whole genome DNA and the construction of fingerprinting were all better. A total of 135 DNA bands were obtained by RAPD-PCR amplification of the tested strains, of which 124 were polymorphic and the polymorphism was 91.9%. NTSYSpc Version2.1 software was used to cluster the genetic distance of the tested strains and the phylogenetic tree was constructed. Based on the genetic similarity coefficient 0.81 as the threshold value, 96 tested strains were divided into 12 RAPD groups, and most of the isolates were inherited Low similarity, significant genetic variation at DNA level and rich genetic diversity. The genetic differentiation of strains in different regions was different. The genetic differentiation of the strains isolated from cucumber was significantly higher than those isolated from melon. There was no significant correlation between RAPD population and geographic origin, host separation, pathogenicity, mating type and metalaxyl resistance.