论文部分内容阅读
目的:检测乳腺癌患者红细胞膜n-6/n-3多不饱和脂肪酸比率的水平,探讨其与乳腺癌临床病理特征的关系。方法:采用气相色谱-质谱联用法检测68例乳腺癌患者,30例乳腺良性病变患者和30名正常健康志愿者血液红细胞膜的n-6/n-3多不饱和脂肪酸的水平,分析n-6/n-3多不饱和脂肪酸比率与乳腺癌临床病理参数的相关性。结果:乳腺癌患者红细胞膜n-6/n-3脂肪酸比率为8.73±0.64,高于乳腺良性病变者的8.41±0.54,t=0.32,P<0.05,也高于正常健康志愿者的3.20±0.75,t=5.53,P<0.01,Ⅲ+Ⅳ期患者(8.89±0.62)高于Ⅰ+Ⅱ期患者(8.54±0.61,t=-2.345,P=0.022),远处转移患者(9.38±0.23)高于无远处转移患者(8.57±0.60,t=-4.742,P<0.01),有腋窝淋巴结转移患者(9.15±0.43)高于无淋巴结转移患者(8.48±0.61,t=-4.886,P<0.01)。结论:乳腺癌组织红细胞膜n-6/n-3脂肪酸比率水平较高,可能在乳腺癌的发生发展过程中发挥重要作用;其与乳腺癌临床分期、远处转移和淋巴结转移相关。
Objective: To detect the level of n-6 / n-3 polyunsaturated fatty acid in erythrocyte membrane in patients with breast cancer and to explore its relationship with clinicopathological features of breast cancer. Methods: The levels of n-6 / n-3 polyunsaturated fatty acids in blood erythrocyte membrane of 68 breast cancer patients, 30 benign breast lesions and 30 healthy volunteers were detected by gas chromatography-mass spectrometry (GC-MS) 6 / n-3 polyunsaturated fatty acid ratio and clinicopathological parameters of breast cancer. Results: The ratio of n-6 / n-3 fatty acid in erythrocyte membrane was 8.73 ± 0.64 in breast cancer patients, 8.41 ± 0.54 in patients with breast benign lesions, t = 0.32, P <0.05, and also higher than that in healthy volunteers (8.54 ± 0.61, t = -2.345, P = 0.022), distant metastasis (9.38 ± 0.23, P <0.01) in patients with stage Ⅲ + Ⅳ (8.89 ± 0.62) (8.57 ± 0.60, t = -4.742, P <0.01). The patients with axillary lymph node metastasis (9.15 ± 0.43) were higher than those without lymph node metastasis (8.48 ± 0.61, t = -4.886, P <0.01). CONCLUSION: The higher n-6 / n-3 fatty acid ratio in erythrocyte membrane of breast cancer may play an important role in the development of breast cancer. It is associated with clinical stage, distant metastasis and lymph node metastasis of breast cancer.