The temporal-spatial distribution features of prehistoric cultures since the Holocene in Zhejiang region were comparatively analyzed based on GIS spatial analysis. Results show that the prehistoric cultures expanded gradually in this region before 4000 cal. a BP. The notable expansions occurred twice, one in the Majiabang-Hemudu cultural period, the other in the Liangzhu cultural period. Meanwhile, the prehistoric cultures were disseminated from west to east coast along river valleys. After 4000 cal. a BP, as represented by the Maqiao Culture, the distributed area of each prehistoric culture contracted. This is obviously due to the termination of spreading trends to east coast, which was simultaneously accompanied by two different modes of production and economic transitions in the north and south Zhejiang region respectively. The distribution of prehistoric cultures was closely related with Holocene sea-level fluctuations, especially on the banks of Hangzhou Bay, where the distribution changes of prehistoric cultural sites were greatly affected by sea-level changes, with the closest relationships between them. After 7000 cal. a BP, the process of lowered sea-level and regression-epeirogenesis provided wider terrestrial living spaces for prehistoric inhabitants. Based on the comparative analyses of the changes of prehistoric cultures and the environmental evolution information recorded in the Qianmutian subalpine peat of Mt. Tianmu and muddy area on the inner shelf of the East China Sea, it is indicated that the changes of prehistoric cultures were synchronized with environmental changes in Zhejiang region. Before 4000 cal. a BP, the eastward expansion of prehistoric cultures in Zhejiang occurred under the background of the Holocene Optimum, and was the expansion and extension under the joint influences of agricultural civilization and maritime civilization. However, after 4000 cal. a BP, the geographical contraction of prehistoric cultures in Zhejiang occurred under the back-ground of dry-cold climate trend and deterioration of coastal marine environment. It is evidenced from the above fact that the development, expansion and contraction of prehistoric cultures are positively correlated to environmental change. The change of the climatic environment is just the underlying reason for these changes and transitions of production modes and economic forms. Therefore, the climatic environment is the dominant factor of prehistoric culture vicissitudes in Zhejiang region, which has exerted great influence on distribution, dissemination, expansion and transmutation of the culture. The temporal-spatial distribution features of prehistoric cultures since the Holocene in Zhejiang region were comparatively analyzed based on GIS spatial analysis. Results show that the prehistoric cultures expanded gradually in this region before 4000 cal. A BP. The notable expansions occurred twice, one in the Majiabang-Hemudu cultural period, the other in the Liangzhu cultural period. the Prehistoric cultures were disseminated from west to east coast along river valleys. After 4000 cal. a BP, as represented by the Maqiao Culture, the distributed area of ​​each This is obviously due to the termination of spreading trends to the east coast, which was when accompanied by two different modes of production and economic transitions in the north and south Zhejiang region respectively. The distribution of prehistoric cultures was closely related with Holocene sea-level fluctuations, especially on the banks of Hangzhou Bay, where the distributio n changes of prehistoric cultural sites were greatly affected by sea-level changes, with the closest relationships between them. After 7000 cal. a BP, the process of lowered sea-level and regression-epeirogenesis provided for terrestrial living spaces for prehistoric inhabitants. Based on the comparative analyzes of the changes of prehistoric cultures and the environmental evolution information recorded in the Qianmutian subalpine peat of Mt. Tianmu and muddy area on the inner shelf of the East China Sea, it is indicated that the changes of prehistoric cultures were synchronized with environmental changes in Zhejiang region. Before 4000 cal. a BP, the eastward expansion of prehistoric cultures in Zhejiang occurred under the background of the Holocene Optimum, and was the expansion and extension under the joint influences of agricultural civilization and maritime civilization. However, after 4000 cal. A BP, the geographical contraction of prehistoric cultures in Zhejiang occurred under the back-ground of dry-cold climate trend and deterioration of coastal marine environment. It is evidenced from the above fact that the development, expansion and contraction of prehistoric cultures are positively correlated to environmental change. The change of the climatic environment is just the underlying reason for these changes and transitions of production modes and economic forms. Therefore, the climatic environment is the dominant factor of prehistoric culture vicissitudes in Zhejiang region, which has exerted great influence on distribution, dissemination, expansion and transmutation of the culture.