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目的验证前列腺素E2(PGE2)、前列腺素F2α(PGF2α)作为脉络膜脱离型视网膜脱离的特征性分子标志物的意义。方法收集2014年9月—2015年9月南京医科大学附属无锡第二人民医院眼科的56例原发性病例,包括26例脉络膜脱离型孔源性视网膜脱离(RRDCD)患者及30例孔源性视网膜脱离(RRD)患者,对玻璃体液分别进行前列腺素E2、F2α酶联免疫吸附试验(ELISA)分析。结果 RRDCD组玻璃体液中PGE2含量低于RRD组,组间差异具有统计学意义(P<0.01),而RRDCD组玻璃体液中PGF2α与RRD组相比差异无统计学意义(P=0.865)。RRDCD患者不同PVR等级(A、B、C、D)玻璃体液中PGE2、PGF2α浓度,差异均无统计学意义(P=0.409、0.389)。将RRDCD患者轻度PVR(A、B)合并后与严重PVR(C、D)比较,玻璃体液的PGE2、PGF2α浓度水平差异均无统计学意义(P=0.433、0.948)。结论 RRDCD患者玻璃体液中PGE2水平低于RRD组,与前期研究结果一致,可能是PGE2在诱发炎症的过程中消耗过多,或者是限制非特异性炎症的应答丧失,导致RRDCD的炎症程度较RRD严重,且PGE2并不随PVR的严重程度而发生变化。而PGF2α在本次研究中没有表现出具有RRDCD分子标志物的潜能。
Objective To investigate the significance of prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) as characteristic molecular markers for choroidal detachment retinal detachment. Methods Fifty-six cases of primary ophthalmology from Wuxi Second People’s Hospital Affiliated to Nanjing Medical University from September 2014 to September 2015 were collected, including 26 patients with rhegmatogenous retinal detachment (RRDCD) and 30 patients with rhegmatogenous Patients with retinal detachment (RRD) were subjected to prostaglandin E2 and F2α enzyme-linked immunosorbent assay (ELISA) on vitreous humor, respectively. Results The content of PGE2 in vitreous humor of RRDCD group was lower than that of RRD group, the difference was statistically significant (P <0.01). There was no significant difference between RRDCD group and RRD group (P = 0.865). There were no significant differences in the concentrations of PGE2 and PGF2α in vitreous humor between different PVR grades (A, B, C, D) in patients with RRDCD (P = 0.409,0.389). There was no significant difference in the levels of PGE2 and PGF2α in vitreous humor between mild PVR (A, B) and severe PVR (C, D) in patients with RRDCD (P = 0.433,0.948). Conclusions The level of PGE2 in vitreous humor of patients with RRDCD is lower than that of RRD group. Consistent with the previous study, PGE2 may be over-consumed in the process of inducing inflammation or loss of response to nonspecific inflammation, resulting in more severe RRDCD than RRD , And PGE2 does not change with the severity of PVR. However, PGF2α did not show the potential of having RRDCD molecular markers in this study.