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目的观察番茄红素对SD大鼠在体心脏缺血再灌注损伤的影响,并探求其作用机制。方法 SD大鼠随机分为假手术组,缺血再灌注损伤组,番茄红素5、15 mg/kg+缺血再灌注损伤组。番茄红素组术前15 d开始ig给药,其他组给予生理盐水,1次/d。制备大鼠缺血再灌注损伤模型后再灌3 h,收集血液,测定血清中心肌酶谱相关指标肌酸激酶(CK)和乳酸脱氢酶(LDH)、氧化应激相关指标超氧化物歧化酶(SOD)和丙二醛(MDA),以及炎症相关指标肿瘤坏死因子α(TNF-α)和白介素1β(IL-1β)的水平,Western blotting法检测心肌中生存素的表达。再灌24 h后取心脏,依文思蓝–氯化三苯基四氮唑双染色测定心肌梗死面积。结果与缺血再灌注损伤组比较,番茄红素可显著减小心肌缺血再灌注损伤后心肌梗死面积(P<0.05),显著降低缺血再灌注损伤后血清中CK、LDH水平(P<0.05),显著升高缺血再灌注损伤后血清中SOD水平而降低MDA水平(P<0.05),显著降低缺血再灌注损伤后血清中TNF-α和IL-1β水平(P<0.05),显著逆转缺血再灌注损伤下调的生存素表达(P<0.05)。结论番茄红素对SD大鼠在体心肌缺血再灌注损伤具有保护作用,其机制与减轻缺血再灌注损伤后氧化应激损伤、抑制炎症反应和激活生存素通路有关。
Objective To observe the effect of lycopene on SD rat myocardial ischemia-reperfusion injury and explore its mechanism. Methods SD rats were randomly divided into sham-operation group, ischemia-reperfusion injury group, lycopene 5,15 mg / kg + ischemia-reperfusion injury group. The lycopene group began ig administration 15 days before the operation and the other groups received normal saline once a day. The model of ischemia-reperfusion injury in rats was prepared and then reperfused for 3 h. Blood samples were collected for determination of creatine kinase (CK) and lactate dehydrogenase (LDH), serum oxidase-related index superoxide dismutase (SOD), malondialdehyde (MDA) and inflammation-related indicators of tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) were detected by immunohistochemistry. Survivin expression in myocardium was detected by Western blotting. After 24 hours of reperfusion, the heart was taken and the area of myocardial infarction was determined by Evans blue-triphenyltetrazolium chloride double staining. Results Compared with ischemia reperfusion injury group, lycopene could significantly reduce myocardial infarct size after myocardial ischemia / reperfusion injury (P <0.05), and significantly reduce serum CK and LDH levels after ischemia / reperfusion injury (P < 0.05). The level of serum SOD and the level of MDA were significantly increased (P <0.05), and the levels of TNF-α and IL-1β in serum were significantly decreased after ischemia / reperfusion (P <0.05) Significantly reverse the expression of survivin down-regulated after ischemia-reperfusion injury (P <0.05). Conclusions Lycopene has a protective effect on myocardial ischemia-reperfusion injury in SD rats, and its mechanism is related to reducing oxidative stress injury, inhibiting inflammatory reaction and activating survivin pathway after ischemia-reperfusion injury.