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自从单克隆抗体问世以来,许多学者利用免疫组化技术对肿瘤局部的浸润单个核细胞(MNC)的分布进行了原位表型分析,但因淋巴细胞和单核/巨噬细胞在功能上的高度不均一性和各个细胞的多功能性,无法根据表型分析结果预知其功能。我们最近应用一组抗IL—受体、IL—1、TNFα和IEN—γ的单(或多)克隆抗体,对15例胃肠癌浸润MNC进行了原位免疫组织化分析。在实验中、我们以经PHA刺激48h后的外周血及炎性胸腹水MNC做为阳性对照,用双重免疫组化技术证明大多数LeuM5~+细胞同时为IL—1~+、
Since the advent of monoclonal antibodies, many scholars have used immunohistochemistry in situ phenotypic analysis of the distribution of locally infiltrating mononuclear cells (MNC) in the tumor. However, due to the functional function of lymphocytes and monocytes / macrophages High heterogeneity and versatility of individual cells preclude the prediction of their function based on phenotypic analysis. We recently performed a one-step immunohistochemical analysis of 15 patients with infiltrating MNCs from gastrointestinal cancers using a panel of single (or polyclonal) antibodies against IL-Receptor, IL-1, TNFα and IEN-γ. In the experiment, we use the peripheral blood and inflammatory pleural effusion MNC 48h after PHA stimulation as a positive control, using double immunohistochemistry to prove that most LeuM5 ~ + cells at the same time as IL-1 ~ +,