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本文采用ELISA间接法对30对不明原因的不育夫妇血清及生殖道分泌液中ASA的阳性率、种类(IgG、IgM、IgA)及分布情况进行了研究。男性病人组血清中ASA阳性率为13.3%,精浆中为16.7%,和对照组相比无统计学意义。女性病人组血清中ASA阳性率为23.3%,宫颈粘液中为33.3%,和对照组相比均有显著差异。在不明原因的不育夫妇中,ASA主要发生在女方。检测结果表明,血清、精浆和宫颈粘液中均存在有IgG、IgM、IgA类ASA。但血清中主要为IgG、IgM类ASA,精浆及宫颈粘液中主要为IgA、IgG类ASA。ASA可同时存在于血清或生殖道分泌液中,也可单独存在于血清或生殖道分泌液中。
In this study, the indirect immunosorbent assay (ELISA) was used to detect the positive rate and genotype (IgG, IgM, IgA) and distribution of ASA in serum and genital secretions of 30 unexplained infertile couples. Serum ASA positive rate was 13.3% in male patients and 16.7% in seminal plasma, which was not statistically significant compared with the control group. Serum ASA positive rate was 23.3% in female patients and 33.3% in cervical mucus, which was significantly different from the control group. In unexplained infertility couples, ASA occurs mainly in the woman. Test results show that there are IgG, IgM, IgA ASA in serum, seminal plasma and cervical mucus. However, the main serum IgG, IgM ASA, seminal plasma and cervical mucus mainly IgA, IgG ASA. ASA can exist in the serum or genital tract secretions, but also exist in the serum or genital tract secretion alone.