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目的 探讨新生儿缺氧缺血性脑病 (HIE)外周血IL - 6、TNF -α与NO变化的临床意义。方法 分别用放射免疫法与硝酸盐还原酶两点法于生后第 1天 (2 4小时内 )、3天与 7天检测了 40例HIE患儿及 40例正常新生儿外周血IL - 6、TNF -α与NO水平的变化。结果 HIE患儿和正常新生儿生后第 1天血清IL - 6水平分别为(5 2 6± 2 4 5 )和 (80 2± 2 9 4)ng/L(两者比较 P <0 0 1) ,TNF -α分别为 (1 18± 0 31)和 (0 91± 0 30 ) μg/L(P<0 0 1) ,NO分别为 (70 3± 32 7)和 (89 2± 35 9) μmol/L(P <0 0 5 ) ,而且病情越重改变越明显。至生后 1周IL - 6、TNF -α恢复至正常对照组水平 ,而NO则逐渐增高 ,至生后 1周超过对照组水平 (P <0 0 1)。结论 HIE患儿外周血IL - 6与NO水平减低 ,TNF -α水平升高 ,它们可能参与了新生儿缺氧缺血性脑损伤的某些发病过程 ;本研究为HIE的免疫学治疗提供了理论依据
Objective To investigate the clinical significance of changes of IL - 6, TNF - α and NO in peripheral blood in neonates with hypoxic - ischemic encephalopathy (HIE). Methods The levels of IL - 6 in peripheral blood of 40 neonates with HIE and 40 normal neonates were detected by radioimmunoassay and nitrate reductase two - point method on the first day after birth (within 24 hours), three days and seven days respectively. , TNF-α and NO levels. Results The serum levels of IL - 6 on the first day after birth in HIE children and normal newborns were (526 ± 2 45) and (80 2 ± 294) ng / L, respectively (P <0.01 ) And TNF-α were (1 18 ± 0 31) and (0 91 ± 0 30) μg / L, respectively (P 0 01), and the levels of NO were 70 3 ± 32 7 and 89 2 ± 35 9 ) μmol / L (P <0 05), and the more serious changes in the more obvious. The level of IL - 6 and TNF - α returned to normal level one week after birth, while the level of NO increased gradually and reached the level of control one week later (P <0.01). Conclusion The levels of IL - 6 and NO in peripheral blood of HIE children are lower and the levels of TNF - α are higher, which may be involved in some pathogenesis of neonatal hypoxic - ischemic brain damage. This study provides the immunological treatment for HIE Theoretical basis