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对不同时期感染甜菜花叶病毒(BtMV)的叶片,进行病理变化系列观察。早期侵染(7天前)的细胞质、细胞器均无变化,随着症状发展,风轮状内含体、束状内含体、细胞核卫星体,细胞质泡囊化旁类似病毒的束状结构,20天达到了高峰,风轮状及束状内含体一直持续两个月还存在。田间样品也观察到了典型的风轮状及束状内含体。细胞核卫星体出现很少。侵染后期,细胞质减少,叶绿体比相应健株提前出现淀粉粒,噬锇颗粒等,基粒片层也提前消解,表现细胞提前衰老的特征。此外细胞内还有许多健株没有的泡状结构。一种为旁壁体,可能与细胞壁加厚有关;一种为多重泡体的叠加,可能是液泡的吞噬作用加强与外源物质(BtMV)侵入有关;另外还有一种可能是过氧化物酶体或线粒体在病毒诱导下泡囊化,其作用不清楚。
The leaves of infected beet mosaic virus (BtMV) in different stages were observed for pathological changes. There was no change in the cytoplasm or organelles of the early stage of infection (7 days earlier). With the development of symptoms, the bundles of wind-like inclusions, bundles of inclusions, nuclear satellite bodies, cytoplasmic vesicle-like viruses, The peak was reached in 20 days, and the wind-like and bundled inclusions persisted for two months. Field samples also observed typical wind-like and bundle-like inclusions. There are few nuclear satellite bodies. In the later stage of infection, the cytoplasm decreased, chloroplasts appeared starch granules and osmotic osmotic granules earlier than the corresponding healthy plants, and the grana tablets also digested earlier, showing the characteristics of premature aging of cells. In addition there are many cells within the cell bubble structure. One is the side wall body, which may be related to thickening of the cell wall; one is the superposition of multiple vesicles, which may be the phagocytosis of the vacuole to enhance the invasion of foreign substances (BtMV); another may be peroxidase Body or mitochondria in the virus-induced vesicle, its role is not clear.