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本文报告应用国产试剂建立直接固相镧系荧光免疫法检测人甲胎蛋白异质体。结果显示:竞争法检测甲胎蛋白异质体的灵敏度为0.94ng/ml,双位点夹心法检测的灵敏度为0.3ng/ml,测定的变异系数小于10％。
This article reports the use of domestic reagents to establish direct solid phase lanthanum fluorescence immunoassay detection of human alpha-fetoprotein heterogeneous body. The results showed that the sensitivity of the competitive method for detection of alpha-fetoprotein heterogeneity was 0.94 ng / ml, the sensitivity of the two-site sandwich method was 0.3 ng / ml, and the coefficient of variation of the assay was less than 10%.