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喷砂结合酸洗技术(SLA)和羟基磷灰石(HA)沉积技术是2种广泛用于改善钛种植体表面特征的方法。本研究中,用一种简单的沉积方法将一层HA沉积在SLA处理的钛表面(SLA-Ti)。应用扫描电子显微镜(SEM)和原子力显微镜(AFM)观察SLA-Ti和HA覆盖的SLA-钛(HA-Ti)的表面形貌和测量表面粗糙度。同时对2种钛表面的蛋白质吸附,成骨细胞趋化和成骨细胞附着进行分析。结果表明:SLA-Ti表面布满大量规则的微孔,HA-Ti表面在多微孔的背景下覆盖大量规则的微颗粒。与SLA-Ti相比,HA-Ti表面的粗糙度较低。2种钛表面的蛋白吸附均连续均匀,成骨细胞趋化也无差异。在试件培养12和24 h后,发现HA-Ti表面附着的成骨细胞及成骨细胞间联系比SLA-Ti表面更多。这些研究表明HA涂层改善了SLA-Ti钛的表面形貌和促进了体外成骨细胞附着。
Blasting combined with pickling (SLA) and hydroxyapatite (HA) deposition are two widely used methods to improve the surface characteristics of titanium implants. In this study, a layer of HA was deposited on the SLA-treated titanium surface (SLA-Ti) by a simple deposition method. The surface morphology and surface roughness of SLA-Ti and HA-covered HA-Ti were observed by scanning electron microscope (SEM) and atomic force microscope (AFM). Protein adsorption, osteoclast chemotaxis and osteoblast attachment of the two titanium surfaces were also analyzed. The results show that the surface of SLA-Ti is covered with a large number of regular micropores, and the surface of HA-Ti covers a large number of regular microparticles under the background of micropores. Compared with SLA-Ti, HA-Ti surface roughness is low. Both types of titanium surface protein adsorption were uniform, osteoblast chemotaxis no difference. After cultured for 12 and 24 h, the osteoblasts and osteoblasts attached to the surface of HA-Ti were found to have more connections than SLA-Ti surfaces. These studies show that the HA coating improves the surface morphology of SLA-Ti titanium and promotes in vitro osteoblast attachment.