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目前,丝虫病流行病学研究中,还没有一种免疫诊断方法可用于现场评价丝虫病防治措施。分子生物学方法,基因克隆和DNA技术,能提供很好的工具、检测特异性抗体和循环抗原,加速丝虫病免疫诊断的研究。近年来,分子生物学应用于丝虫病免疫诊断研究,已取得了很有希望的结果。已建成了盘尾丝虫、马来丝虫和彭亨丝虫的基因表达库,并对其中若干组基因进行了研究,以重组具有诊断价值的抗原。生化研究结果显示盘尾丝虫和淋巴丝虫的种特异性抗原在浸出液中的低分子部分中,Lobest等用20～
At present, none of the immunodiagnostic methods used in epidemiological studies of filariasis can be used to evaluate filariasis prevention and treatment measures on the spot. Molecular biology methods, gene cloning and DNA technology can provide good tools to detect specific antibodies and circulating antigens, and to accelerate the diagnosis of filariasis immune research. In recent years, molecular biology applied to the diagnosis of filariasis immunization, has achieved very promising results. Gene expression banks for Onchocerca, Malayi and Paenibacillus have been established and several of these genes have been studied to reconstitute diagnostic antigens. Biochemical studies have shown that in the low molecular fraction of species-specific antigens of Onchocerciasis and Lymphatic filariasis in leachate, Lobest et al.