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目的探讨乌贼墨促进免疫低功小鼠免疫功能的作用。方法按0.1,0.25和0.5mL剂量给C57BL/6小鼠灌胃5%的新鲜乌贼墨,每日1次,连续7d,并在给药的第1日和第2日同时腹腔注射环磷酰胺造成免疫功能低下模型,第9日检测小鼠免疫功能的变化。结果乌贼墨可促进免疫低功小鼠的脾淋巴细胞增殖;增强NK细胞杀伤活性;提高免疫效应分子TNF-α,IFN-γ及一氧化氮(NO)的产生水平。结论乌贼墨可增强固有免疫细胞和特异性免疫细胞的活性,调节细胞因子和效应分子的分泌,刺激免疫低功小鼠的免疫功能恢复。
Objective To investigate the effect of sepia on immune function in immunocompromised mice. Methods C57BL / 6 mice were inoculated with 5% fresh sepia at a dose of 0.1, 0.25 and 0.5 mL once daily for 7 days, and intraperitoneal injection of cyclophosphamide on days 1 and 2 of the administration Resulting in immune dysfunction model, the first 9 days to detect changes in immune function in mice. Results Squid ink can promote the proliferation of splenic lymphocytes, enhance the cytotoxic activity of NK cells, and increase the levels of TNF-α, IFN-γ and NO in immunocompromised mice. Conclusion Squid ink can enhance the activity of innate immune cells and specific immune cells, regulate the secretion of cytokines and effector molecules, and stimulate immune function recovery in immunocompromised mice.