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大多数神经退化性疾病,例如帕金森氏症、阿滋海默症等的在分子层面的发病机理目前尚不清楚.大多数蛋白质的表达分析都是在完整组织均质化制备的样品的基础上完成的.用这种方法制备的样品,没有提供特定细胞类型的相关改变的信息.本研究的目的在于检查用激光显微切割方法制备死后脑组织单细胞类型样品能否用于蛋白质表达分析.1 实验部分1.1 样品制备组织样品来自德国慕尼黑路德维希-马克西米利安大学神经病理学系的脑组织库.样品取自死后的人小脑并经快速冷冻,含白质和皮质.样品按神经
At the molecular level, the pathogenesis of most neurodegenerative diseases such as Parkinson’s disease and Alzheimer’s disease is not known at present, and most protein expression analyzes are the basis for samples prepared by homogenization of whole tissues The samples prepared in this way did not provide information on the relevant changes in a particular cell type.The purpose of this study was to examine whether single-cell type samples of brain tissue after death can be used for protein expression analysis by laser microdissection .1 Experimental 1.1 Sample Preparation Tissue samples were obtained from the brain tissue bank of the Department of Neuropathology, Ludwig-Maximilian University, Munich, Germany Sample taken from post-mortem human cerebellum and rapidly frozen with white matter and cortex Samples nerve