论文部分内容阅读
目的探讨Survivin表达在结直肠癌发生,转移中的作用及临床意义。方法 2003年1月-2004年12月间采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连结法测定58例结直肠癌、12例结直肠腺瘤和9例正常结肠组织中Survivin的表达水平,研究其与结直肠癌患者临床病理特征及预后的关系。结果 Survivin蛋白在正常结直肠黏膜中不表达,而在结直肠腺瘤及结直肠癌组织中的表达均高于正常大肠黏膜,差异有统计学意义(P<0.05)。不同年龄、性别、浆膜浸润、淋巴结转移、远处转移、分化程度、Dukes分期等之间Survivin的表达差异均无统计学意义(P>0.05)。Kaplan-Meier生存分析提示:Survivin表达阳性患者的5年生存率为51.2%,阴性者为90.9%,两组比较差异有统计学意义(P<0.05)。结论 Survivin的异常表达参与了结直肠癌发生、发展的病理生理过程,对结直肠癌患者的治疗方式的选择、疗效和预后的评估方面具有重要的参考价值。
Objective To investigate the role and clinical significance of Survivin expression in the pathogenesis and metastasis of colorectal cancer. METHODS: Survivin in 58 cases of colorectal cancer, 12 cases of colorectal adenoma and 9 cases of normal colon tissues were determined by immunohistochemical streptavidin-peroxidase method from January 2003 to December 2004. Expression level, to study its relationship with clinicopathological features and prognosis of patients with colorectal cancer. Results Survivin protein was not expressed in normal colorectal mucosa, but higher in colorectal adenoma and colorectal cancer tissues than in normal colorectal mucosa, the difference was statistically significant (P <0.05). There was no significant difference in the expression of Survivin between different age, gender, serosal invasion, lymph node metastasis, distant metastasis, differentiation, Dukes stage and so on (P> 0.05). The Kaplan-Meier survival analysis indicated that the 5-year survival rate of patients with positive Survivin expression was 51.2% and that of negative patients was 90.9%. There was significant difference between the two groups (P <0.05). Conclusion The abnormal expression of Survivin is involved in the pathophysiological process of the occurrence and development of colorectal cancer. It has important reference value for the selection of treatment modalities and prognosis in patients with colorectal cancer.