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抗性基因转移是细菌形成耐药性的重要原因。近年来的研究表明胁迫因子可通过多种机制诱导抗性基因转移。DNA损伤可导致细菌产生SOS应激反应,进而诱导接合DNA介导的抗性基因转移。在一些缺乏SOS系统的细菌中,抗生素胁迫可诱导细菌建立自然转化感受态。此外,作者最近的研究表明普通胁迫应答因子RpoS调控一种由双链质粒DNA介导的固体基质表面的抗性基因转移方式。本文在总结SOS依赖和非依赖型胁迫因子诱导细菌接合和转化介导的DNA转移以及RpoS调控固体基质表面双链质粒DNA转移的基础上,提出今后需重点研究胁迫因子如何激活关键调控蛋白以及这些调控蛋白如何影响DNA转移相关基因表达等关键问题。解决上述问题将为寻找合适的分子靶标用于防控抗性基因转移导致的细菌耐药奠定基础。
Resistance gene transfer is an important cause of bacterial resistance. Recent studies have shown that stress factors can induce resistance gene transfer through a variety of mechanisms. DNA damage can lead to bacterial SOS stress response, which in turn leads to DNA-mediated resistance-mediated gene transfer. In some bacteria lacking the SOS system, antibiotic stress induces bacteria to establish naturally-transformed competent states. In addition, recent studies by the authors show that RpoS, a common stress response factor, regulates a gene-transfer-resistant manner on solid substrate surfaces mediated by double-stranded plasmid DNA. In this review, we summarize the DNA transfer induced by SOS-dependent and independent stress-induced bacterial conjugation and transformation and the regulation of double-stranded plasmid DNA transfer by RpoS on the surface of solid matrix. It is suggested that in the future, we will focus on how stress factors activate key regulatory proteins and these How regulatory proteins affect DNA transfer-related gene expression and other key issues. Solving the above problems will lay the foundation for finding suitable molecular targets for preventing and controlling bacterial resistance caused by resistance gene transfer.