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Ⅰ型多发性神经纤维瘤基因(NF 1)最近已被定位于17号染色体靠近着丝粒的区域。进一步的连锁分析确定了NF 1两侧的标记位点和与之紧密连锁的标记位点。本文报道了英国及南美洲患NF 1家族中17号染色体的9个标记位点的分析。本资料研究的样本包括144个有信息意义的减数分裂相和20个NF1家族。这些NF1家族其中16个是英国的、4个是印裔南非的、1个是居住南非的欧洲家族。关于NF1的诊断指标和临床症状见于其他的资料。用常规方法对DNA进行提取、分离、限制酶切、电泳、转移,与放射标记的探针杂交,对NF 1家族中17号染色体上的下述标
Type I multiple neurofibroma gene (NF 1) has recently been located on chromosome 17 near the centromere region. Further linkage analysis identified the marker sites flanking the NF 1 and closely linked marker sites. This article reports the analysis of nine marker sites on chromosome 17 in the NF 1 family in England and South America. The sample data for this study include 144 informative meiotic and 20 NF1 families. Sixteen of these NF1 families are British, four are Indo-South Africa and one is a European family living in South Africa. The diagnostic criteria and clinical symptoms of NF1 are found in other sources. The DNA was extracted, isolated, restricted, electrophoresed, and transferred by conventional methods and hybridized to radiolabeled probes. The following subscripts on chromosome 17 in the NF 1 family