为鉴定转导SaNHX耐盐基因T1代980个红麻株系的耐盐性,本研究对980个株系在福州进行耐盐性鉴定和筛选,并对筛选出的9个耐盐T1代株系进行PCR检测和不同盐浓度水培和盆栽初步鉴定。通过调查转基因T1代在盐胁迫条件下的发芽率、出苗率、幼苗生长情况,对转基因后代的耐盐水平进行评价。结果表明:(1)转耐盐基因红麻T1代9个株系在0.71%的处理下,种子发芽率和生长势明显优于未转导对照红麻。苗高、苗重和根长分别比对照提高了2.04cm,0.23g和3.7cm。(2)0.3%、0.5%和0.71%三个不同盐浓度胁迫5天、10天、15天三个处理试验的方差分析表明,耐盐转基因T1代的株系幼苗成活率明显高于普通对照,差异均达极显著水平,其中编号176株系最佳;(3)在0.71%盐胁迫处理下,生长10天的T1代红麻幼苗苗高、苗重和根长与对照存在显著差异。在9个转基因株系中,176号植株最好,其苗高、苗重和根长的增长量分别比对照提高5.47cm、0.72g和4.54cm。 In order to identify the salt tolerance of 980 kenaf lines from the T1 generation of SaNHX translocation genes, 980 lines were identified and screened for their salt tolerance in Fuzhou. Nine salt tolerant T1 lines Department of PCR detection and different salt concentrations of hydroponics and pot preliminary identification. The salt tolerance of transgenic offspring was evaluated by investigating the germination rate, germination rate and seedling growth of transgenic T1 generation under salt stress conditions. The results showed that: (1) The seed germination rate and growth potential of nine strains of T1-resistant salt-tolerant kenaf were better than those of untransfected kenaf under the treatment of 0.71%. Seedling height, seedling weight and root length were increased by 2.04cm, 0.23g and 3.7cm respectively. (2) The ANOVA of three treatments with 0.3%, 0.5% and 0.71% stress for 5 days, 10 days and 15 days showed that the survival rate of seedlings of T1 transgenic lines was significantly higher than that of control , And the difference reached the extremely significant level, of which the number 176 was the best. (3) Under the treatment of 0.71% salt stress, the height, seedling weight and root length of T1 generation kenaf seedlings grown for 10 days were significantly different from the control. Among the nine transgenic lines, plant 176 was the best, and its seedling height, seedling weight and root length increased by 5.47cm, 0.72g and 4.54cm, respectively.