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本文报导以人活化Ｂ淋巴细胞的３Ｄ５细胞的ｍＲＮＡ为模板，逆转录合成ｃＤＮＡ的第一链：按常规的自身引物法，以合成的第一链为模板合成ｃＤＮＡ第、二链；以及λｇｔ１１Ｓｆｉ－Ｎｏｔ噬菌体为载体，定向插入构建了人活化Ｂ细胞。ＤＮＡ表达文库。该文库的大小为２．５×１０６ｐｆｕ，插入片段长度大于１ｋｂ；用抗ＣＤ７１及ＣＤ９８单克隆抗体为探针，对所构建的文库进行筛选，均获得了阳性克隆，ＣＤ７１及ＣＤ９８均为人Ｂ细胞活化时才得以表达的分化抗原，从而证明，新构建的ｃＤＮＡ文库是人活化Ｂ细胞的ｃＤＮＡ表达文库。
In this paper, the mRNA of human activated B lymphocytes in 3D5 cells was used as a template to reverse transcribe the first strand of cDNA: The first and second strands of cDNA were synthesized according to the conventional first-strand method as a template, and the λgt11Sfi- Not phage as a vector, directional insert constructed human activated B cells. DNA expression library. The size of the library was 2.5 × 106pfu and the length of inserted fragment was more than 1kb. Positive clones were obtained by screening the constructed libraries using anti-CD71 and CD98 monoclonal antibodies as probes. Both CD71 and CD98 were human B cells Activated when they were able to express differentiation of the antigen, thus demonstrating that the newly constructed cDNA library is a human activated B cell cDNA expression library.