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目的探索塞来昔布对细粒棘球蚴原头节体外生长及p-ERK2表达的影响。方法实验分组为1640培养基组、DMSO组、塞来昔布溶液(50、100、150、200μmol/L)中体外孵育,在倒置焦镜下观察原头节的形态变化,同时通过0.1%伊红染色显示原头节活力。运用Western blot检测DMSO、100、150、200μmol/L塞来昔布处理细粒棘球蚴原头节48h后p-ERK2蛋白的表达量。结果体外孵育7d后,DMSO组及空白1640对照组原头节的活力几乎没有改变。塞来昔布作用1d后,100、150、200μmol/L组原头节的活力均下降;作用3d后,200μmol/L组原头节全部被杀死,150μmol/L组的原头节活力为43.9%。100μmol/L、50μmol/L组对原头节的活力也有明显下降,但不及高浓度组显著。Western blot结果显示P-ERK2的表达量随浓度增加而下降趋势明显。结论塞来昔布能够抑制细粒棘球蚴原头节生长,这可能与塞来昔布下调P-ERK2的表达进而抑制ERK2信号传导通路有关。
Objective To investigate the effect of celecoxib on the growth and expression of p-ERK2 in vitro of protoscoiia of Echinococcus granulosus. Methods The experiment groups were incubated in vitro in DMSO group and celecoxib group (50, 100, 150, 200μmol / L) in 1640 medium group. Morphological changes were observed under inverted focal microscope. At the same time, Red staining shows the vitality of the original head. Western blot was used to detect the expression of p-ERK2 in DMSO, 100, 150 and 200μmol / L celecoxib for 48 hours. Results After 7 days of in vitro incubation, the viability of protoscoleces in DMSO group and control group was almost unchanged. After being treated with celecoxib for 1 day, the viability of the protoscoleces in 100, 150 and 200 μmol / L groups decreased. After 3 days of treatment, the protoscoleces in 200 μmol / L group were all killed. The protoscoleces in 150 μmol / L group were 43.9%. 100μmol / L, 50μmol / L group of the original head of vitality also significantly decreased, but not as high concentration group significantly. Western blot results showed that the expression of P-ERK2 decreased with increasing concentration. Conclusion Celecoxib can inhibit the growth of protoscolex nodosum of Echinococcus granulosus, which may be related to the down-regulation of P-ERK2 expression and the inhibition of ERK2 signal transduction pathway.