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目的：通过对Fas、FasL和caspase的蛋白表达及与细胞凋亡关系的观察，探讨宫颈鳞状细胞癌组织中细胞凋亡的机制。方法：应用原位末端标记法（TUNEL）、双重荧光染色法等检测5例正常宫颈组织和34例不同分化程度的宫颈鳞状细胞癌组织的细胞凋亡和Fas、FasL、caspase－3、caspase－8蛋白的表达。结果：宫颈正常及鳞癌组织上有细胞凋亡及Fas、FasL、caspase－3蛋白的表达，各指标之间有相关性。癌组织中，Fas、FasL、caspase－3蛋白阳性细胞明显增多，双重荧光染色显示，部分细胞Fas及cspase－3蛋白与凋亡细胞同时阳性染色。宫颈正常及癌组织中无caspase－8蛋白表达。结论：正常宫颈及宫颈鳞癌组织中，细胞凋亡处于动态调整过程中，Fas／FasL可能通过活化caspase－3参与细胞凋亡的调节。
OBJECTIVE: To investigate the mechanism of apoptosis in squamous cell carcinoma of the cervix by observing the protein expression of Fas, FasL and caspase and its relationship with apoptosis. Methods: The apoptosis, Fas, FasL, caspase-3 and caspase were detected by TUNEL and double fluorescence staining in 5 cases of normal cervical tissue and 34 cases of cervical squamous cell carcinoma with different differentiation degree -8 protein expression. Results: Apoptosis and expression of Fas, FasL and caspase-3 proteins in normal and squamous cell carcinoma of cervix were correlated with each other. The positive cells of Fas, FasL and caspase-3 in cancer tissues were significantly increased, and the positive staining of Fas and cspase-3 protein in some cells was positive staining with apoptotic cells by double fluorescence staining. Cervical normal and cancerous tissue without caspase-8 protein expression. Conclusion: In normal cervical and cervical squamous cell carcinoma, apoptosis is under dynamic regulation. Fas / FasL may be involved in the regulation of apoptosis by activating caspase-3.