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Objective: To investigate the reversal effect of arsenic trioxide (As2O3) on the multidrug resistance of human gas-tric tumor SGC7901/ADR cell line to adriamycin (ADM) and its reversal mechanisms. Methods: The non-cytotoxic concentra-tion of As2O3 and the sensitivity of SGC7901/ADR cells to ADM were detected by MTT assay. The drug concentration and P-gp function of SGC7901/ADR cells were measured with flow cytometry (FCM), and the impacts of As2O3 on the GST-π and Topo II expressions of SGC7901/ADR cells were analyzed by immunohistochemical method. Results: As2O3 at 0.4 to 0.8 μmol/L concentrations were not significantly cytotoxic to SGC7901/ADR cells. As2O3 at 0.8 μmol/L could improve the sensitivity of SGC7901/ADR cells to ADM via inhibiting P-gp function, down-regulating GST-π expression and increasing the intracellular accumulation of ADM in SGC7901/ADR cells. Conclusion: As2O3 can reverse partly the drug-resistance of SGC7901/ADR cells to ADM, which may be related with inhibiting the P-gp function and down-regulating GST-π expression.
Objective: To investigate the reversal effect of arsenic trioxide (As2O3) on the multidrug resistance of human gas-tric tumor SGC7901 / ADR cell line to adriamycin (ADM) and its reversal mechanisms. Methods: The non-cytotoxic concentra tion of As2O3 and the sensitivity of SGC7901 / ADR cells to ADM were detected by MTT assay. The drug concentration and P-gp function of SGC7901 / ADR cells were measured with flow cytometry (FCM), and the impacts of As2O3 on the GST-π and Topo II Expression of SGC7901 / ADR cells were analyzed by immunohistochemical method. Results: As2O3 at 0.4 to 0.8 μmol / L concentrations were not significantly cytotoxic to SGC7901 / ADR cells. As2O3 at 0.8 μmol / L could improve the sensitivity of SGC7901 / ADR cells to ADM via inhibiting P-gp function, down-regulating GST-π expression and increasing the intracellular accumulation of ADM in SGC7901 / ADR cells. Conclusion: As2O3 can reverse partially the drug-resistance of SGC7901 / ADR cells to ADM, which may be related with inhibit ing the P-gp function and down-regulating GST-π expression.