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该文应用胶质银染技术、自动图像分析仪DNA含量测量技术对56例大肠癌手术标本进行了检测。结果发现Ag－NOR计数与患者存活时间呈负相关（r＝－0．77P＜0．001）。Ag－NOR计数低，患者存活时间长，预后好；反之则预后差。对数秩时序检验P＜0．001。在DNA含量总体直方图中，大肠癌细胞DNA主峰位于2～6C之间，预后好组，其主峰左移至2C附近，大于5C细胞群较少（10．48±9．89），而预后差组主峰右移至5C附近，大于5C细胞所占比例明显增多（61．59±21．45）。2C细胞群为主大肠癌Ag－NOR计数（4．04±0．66）低于以非整倍体细胞群占多数大肠癌（5．92±1．49）（P＜0．01）。结合运用上述指标可提高判断预后的准确性。
In this paper, colloidal silver staining technique and automatic image analyzer DNA content measurement technique were used to detect 56 colorectal cancer surgical specimens. The results showed a negative correlation between Ag-NOR count and survival time (r=-0.77P<0.001). Ag-NOR counts are low, patients have long survival time, and the prognosis is good; otherwise, the prognosis is poor. Log rank test P<0.001. In the overall histogram of DNA content, the main DNA of colorectal cancer cells is located between 2 and 6C, and the prognosis is good. The main peak shifts to the left near 2C, and the number of cells larger than 5C is smaller (10.48±9.89). The main peak of the difference group shifted to the right near 5C, and the proportion of cells with greater than 5C cells increased significantly (61.59±21.45). The Ag-NOR count (4.04±0.66) in the 2C cell population-based colorectal cancer was lower than that in the aneuploid cell population (5.92±1.49) (P<0.01). Combined use of the above indicators can improve the accuracy of prognosis.