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分别利用苗期人工接种鉴定及ELISA检测方法,对127份不结球白菜种质资源进行芜菁花叶病毒(TuMV)的抗性鉴定。结果显示,苗期人工接种鉴定的病情指数(DI)分布在3.55~95.68之间,不同种质间表现出较大的抗性差异。不同抗性级别的次数分布图基本符合正态分布,略向感病区域偏离。基于病情指数的聚类分析结果与抗病性分级基本一致。通过苗期鉴定共筛选获得高抗TuMV的不结球白菜种质6份,抗病种质13份,其主要农艺性状表现出一定的多样性。其中叶面皱缩和具有刺毛的抗病材料所占比例较高,可能与TuMV抗性存在一定的相关性。ELISA检测结果显示,117份供试种质的P/N值分布在3.10~25.37之间,不同种质间表现出病毒含量的差异,但与DI值未呈现明显相关性,可作为抗病材料筛选的辅助指标。
Resistance of turnip mosaic virus (TuMV) to 127 non-heading Chinese cabbage germplasms was identified by artificial inoculation and ELISA assay respectively. The results showed that the disease index (DI) of artificial inoculation identification at seedling stage ranged from 3.55 to 95.68, showing significant differences among different germplasms. The distribution maps of different resistance levels basically conform to the normal distribution, slightly deviate from the susceptible area. The results of cluster analysis based on disease index were basically consistent with the disease resistance classification. Six seedlings of non-heading Chinese cabbage with high resistance to TuMV and 13 disease-resistant germplasms were screened through seedling identification. Their main agronomic traits showed some diversity. Among them, the proportion of leaf-surface shrinkage and the disease-resistant material with bristles was higher, which may be related to TuMV resistance. The results of ELISA showed that the P / N values of 117 tested germplasms ranged from 3.10 to 25.37. The differences of virus content among different germplasm showed no significant correlation with DI value, which could be used as disease-resistant materials Auxiliary indicator of screening.